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The new pictures show Madeleine smiling and feeding a chocolate lollipop to her younger sister Amelie before she disappeared.
The McCanns, both 41, from Rothley, Leicestershire, said: “Christmas is a time for children. Please help us bring ours back.”
Madeleine was nearly four when she went missing from her family’s holiday apartment in Praia da Luz in Portugal on May 3 2007 while her parents dined with friends nearby.
Despite a massive police investigation and publicity worldwide, she has not been found.
Last weekend Mr and Mrs McCann returned to Praia da Luz together for the first time since Portuguese police named them as suspects in their daughter’s disappearance.
The couple, whose suspect status was later lifted, visited the seaside resort’s church of Nossa Senhora da Luz.
They wandered along the beach where Kate said she felt able to reach out to Madeleine and find a little solace.
Mrs McCann said: “Although our pain feels much rawer here, it is comforting at the same time since we feel closer to Madeleine.”
